BCL9 Antibody (N-Term)

AMM08755G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM08755G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Tel: 010-56256916
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BCL9 Antibody (N-Term)

品牌	Leading Biology	貨號(hào)	AMM08755G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BCL9 antibody (N-Term).
分子量	149290 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
靶點(diǎn)	This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9.
亞型	Rabbit Ig
基因ID	607
UniProt ID	O00512
功能	Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).
總結(jié)	Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, WB, E
稀釋方法	IF~~1:25 WB~~1:2000
別名	B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AMM08755G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: human testis lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 149 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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