品牌 |
Leading Biology | 貨號 |
AMM08728G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality (Mouse) Pou5f1 antibody (N-term).
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分子量 |
38216 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE- ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163}. Note=Expressed in a diffuse and slightly punctuate pattern
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Mouse
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靶點(diǎn) |
This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-119 amino acids from the N-terminal region of Mouse Pou5f1.
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亞型 |
Rabbit Ig
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通用名 |
Oct-3, Oct-4, Otf-3, Otf3
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基因ID |
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UniProt ID |
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功能 |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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總結(jié) |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:2000
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別名 |
POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3
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圖像 |
AMM08728G staining (Mouse) Pou5f1 in mouse brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-Pou5f1 Antibody (N-term)at 1:2000 dilution + F9 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-Pou5f1 Antibody (N-term)at 1:2000 dilution + F9 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說明書 |
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數(shù)量 |
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