(Mouse) Ihh Antibody (N-term)

AMM08712G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM08712G		100 μl / ￥4950

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(Mouse) Ihh Antibody (N-term)

品牌	Leading Biology	貨號	AMM08712G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Ihh antibody (N-term).
分子量	45485 Da
細(xì)胞定位	Antigen Cellular Localization: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side. Note=The N-terminal peptide remains associated with the cell surface. Cell membrane.
宿主	Rabbit
種屬反應(yīng)性	Mouse
靶點	This mouse Ihh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 57-90 amino acids from the N-terminal region of mouse Ihh.
亞型	Rabbit Ig
基因ID	16147
UniProt ID	P97812
功能	Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
總結(jié)	Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IHC-P, WB, FC, E
稀釋方法	IHC-P~~1:25 FC~~1:25 WB~~1:2000
別名	Indian hedgehog protein, IHH, HHG-2, Indian hedgehog protein N-product, Indian hedgehog protein C-product, Ihh
圖像	AMM08712G staining (Mouse) Ihh in mouse kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing Jurkat cells stained with AMM08712G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12735b, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-Ihh Antibody (N-term) at 1:2000 dilution + mouse kidney lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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