GNL3 Antibody (N-term)

APR17769G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR17769G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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GNL3 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR17769G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GNL3 antibody (N-term).
分子量	61993 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the N-terminal region of human GNL3.
亞型	Rabbit Ig
通用名	E2IG3, NS
基因ID	26354
UniProt ID	Q9BVP2
功能	May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
總結(jié)	May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	IF~~1:25 WB~~1:2000
別名	Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling GNL3 with APR17769G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red). All lanes : Anti-GNL3 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說(shuō)明書(shū)	Product Datasheet Protocol
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