(Mouse) Cldn6 Antibody (C-term)

APR10975G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR10975G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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(Mouse) Cldn6 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR10975G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Cldn6 antibody (C-term).
分子量	23388 Da
細(xì)胞定位	Antigen Cellular Localization: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein
宿主	Rabbit
種屬反應(yīng)性	Mouse
靶點(diǎn)	This Mouse Cldn6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 186-229 amino acids from the C-terminal region of mouse Cldn6.
亞型	Rabbit Ig
基因ID	54419
UniProt ID	Q9Z262
功能	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.
總結(jié)	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity (By similarity).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	FC~~1:25 WB~~1:1000
別名	Claudin-6, Skullin, Cldn6
圖像	Overlay histogram showing colon26 cells stained with APR10975G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR10975G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term)( APR10975G). APR10975G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
說(shuō)明書	Product Datasheet Protocol
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