品牌 |
Leading Biology | 貨號 |
APR07558G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DHFR antibody (N-term).
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分子量 |
21453 Da
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse, Rat
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靶點 |
This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
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總結(jié) |
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:1000
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別名 |
Dihydrofolate reductase, DHFR
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圖像 |
Immunohistochemical analysis of paraffin-embedded H. liver section using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Immunohistochemical analysis of paraffin-embedded H. brain section using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from 293T, Hela, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate(from left to right), using DHFR Antibody (N-term)( APR07558G). APR07558G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
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說明書 |
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數(shù)量 |
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