品牌 |
Leading Biology | 貨號(hào) |
APR08636G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDUA4 antibody (C-term).
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分子量 |
9370 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
56-80 aa
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靶點(diǎn) |
This NDUA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-80 amino acids from the C-terminal region of human NDUA4.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance.
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總結(jié) |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, IHC-P, E
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稀釋方法 |
IHC-P~~1:25
IF~~1:25
WB~~1:1000
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別名 |
Cytochrome c oxidase subunit NDUFA4, Complex I-MLRQ, CI-MLRQ, NADH-ubiquinone oxidoreductase MLRQ subunit, NDUFA4
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圖像 |
Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUA4 Antibody (C-term)( AP20519B). AP20519B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Fluorescent image of Hela cells stained with NDUA4 Antibody (C-term)( AP20519B). AP20519B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). NDUA4 Antibody (C-term) (Cat. APR08636G) western blot analysis in SW480,Hela cell line lysates (35ug/lane).This demonstrates the NDUA4 antibody detected the NDUA4 protein (arrow). |
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說(shuō)明書 |
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數(shù)量 |
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