LC3 Antibody (APG8B) (N-term)

AMM04363G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM04363G		100 μl / ￥4950

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LC3 Antibody (APG8B) (N-term)

品牌	Leading Biology	貨號	AMM04363G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LC3 antibody (APG8B) (N-term).
分子量	14688 Da
細胞定位	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
宿主	Rabbit
種屬反應性	Human, Rat
免疫原	1-30 aa
靶點	This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
亞型	Rabbit Ig
通用名	MAP1ALC3
基因ID	81631
UniProt ID	Q9GZQ8
功能	Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
總結(jié)	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:100 IHC-P~~1:50~100
別名	Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
圖像	Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (N-term)( AMM04363G)(upper) or Beta-actin (lower). Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat. AMM04363G). U251 cells(right) were treated with Chloroquine (50 μM,16h). AMM04363G was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue). Wild-type (Cln3+/+) or homozygous Cln3?ex7/8 (Cln3?ex7/8/?ex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (Cat. AMM04363G LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3?ex7/8 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer. Data courtesy of Dr. Susan Cotman, Massachusets General Hospital.
說明書	Product Datasheet Protocol
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