ATP5J Antibody (Center)

APG02135G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG02135G		100 μl / ￥4950

大規(guī)格詢價

3 Images

訂購信息

安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
Inquiry@annoron.com

全國授權(quán)代理商

ATP5J Antibody (Center)

品牌	Leading Biology	貨號	APG02135G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP5J antibody (Center).
分子量	12588 Da
細胞定位	Antigen Cellular Localization: Mitochondrion. Mitochondrion inner membrane.
宿主	Rabbit
種屬反應(yīng)性	Mouse, Rat
免疫原	28-56 aa
靶點	This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
亞型	Rabbit Ig
通用名	ATP5A, ATPM
基因ID	522
UniProt ID	P18859
功能	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
總結(jié)	Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the innermembrane during oxidative phosphorylation. It is composed of twolinked multi-subunit complexes: the soluble catalytic core, F1, andthe membrane-spanning component, Fo, which comprises the protonchannel. The F1 complex consists of 5 different subunits (alpha,beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3beta, and a single representative of the other 3. The Fo seems tohave nine subunits (a, b, c, d, e, f, g, F6 and 8). This geneencodes the F6 subunit of the Fo complex, required for F1 and Fointeractions. Alternatively spliced transcript variants encodingdifferent isoforms have been identified for this gene. A pseudogeneexists on chromosome Yp11.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
別名	ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM
圖像	Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody (Center)( APG02135G). APG02135G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. Fluorescent confocal image of U251 cell stained with ATP5J Antibody (Center)( APG02135G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).ATP5J immunoreactivity is localized to Mitochondria significantly. ATP5J Antibody (Center) (Cat. APG02135G) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the ATP5J antibody detected the ATP5J protein (arrow).
說明書	Product Datasheet Protocol
數(shù)量

加入購物車詢價

上一產(chǎn)品：DNAJB11 Antibody (N-term) 下一產(chǎn)品：Phospho-mouse TSC1(S594) Antibody

熱銷產(chǎn)品

產(chǎn)品添加成功！

購物車已有 0 件產(chǎn)品

繼續(xù)訂購查看訂購單

加入詢價單成功！

詢價單已有 0 件產(chǎn)品

繼續(xù)詢價查看詢價單

選擇	品牌	貨號	產(chǎn)品名稱	規(guī)格	分類	研究領(lǐng)域	說明書	數(shù)量	目錄價
1	Leading Biology	APR05963G	EGFR Antibody (Y1092)	100 μl	Polyclonal Antibodies				￥4950.00	訂購詢價
2	Leading Biology	APR03116G	BRCA1 Antibody (aa1847-1863)	100 μl	Polyclonal Antibodies				￥4950.00	訂購詢價
3	Leading Biology	AMM05633G	Bombesin Receptor 2 (extracellular) Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價
4	Leading Biology	APR10986G	AP3B1 Antibody (aa2-14)	50 μg	Polyclonal Antibodies				￥4950.00	訂購詢價
5	Leading Biology	APR11512G	Bestrophin-1 (extracellular) Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價
6	Leading Biology	APG01407G	A3 Adenosine Receptor Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價

最新加入的商品

最新加入的商品

全部產(chǎn)品分類