品牌 |
Leading Biology | 貨號 |
APR07556G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DHCR7 antibody (C-term).
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分子量 |
54489 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Endoplasmic reticulum membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
437-463 aa
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靶點 |
This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.
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亞型 |
Rabbit Ig
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通用名 |
D7SR
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基因ID |
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UniProt ID |
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功能 |
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
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總結(jié) |
This gene encodes an enzyme that removes the C(7-8) doublebond in the B ring of sterols and catalyzes the conversion of7-dehydrocholesterol to cholesterol. This gene is ubiquitouslyexpressed and its transmembrane protein localizes to theendoplasmic reticulum membrane and nuclear outer membrane.Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); asyndrome that is metabolically characterized by reduced serumcholesterol levels and elevated serum 7-dehydrocholesterol levelsand phenotypically characterized by mental retardation, facialdysmorphism, syndactyly of second and third toes, andholoprosencephaly in severe cases to minimal physical abnormalitiesand near-normal intelligence in mild cases. Alternative splicingresults in multiple transcript variants that encode the sameprotein.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IF, WB, IHC-P, E
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稀釋方法 |
IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
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別名 |
7-dehydrocholesterol reductase, 7-DHC reductase, Putative sterol reductase SR-2, Sterol Delta(7)-reductase, DHCR7, D7SR
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圖像 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP11452B at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Li-7 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human testis lysate Lane 5: mouse testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. DHCR7 Antibody (C-term) (Cat. APR07556G) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the DHCR7 antibody detected the DHCR7 protein (arrow). |
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說明書 |
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數(shù)量 |
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