品牌 |
Leading Biology | 貨號 |
APR07788G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HNF4A antibody (N-term).
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分子量 |
52785 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
107-139 aa
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靶點(diǎn) |
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human HNF4A.
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亞型 |
Rabbit Ig
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通用名 |
HNF4, NR2A1, TCF14
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基因ID |
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UniProt ID |
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功能 |
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.
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總結(jié) |
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, E
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稀釋方法 |
WB~~1:1000
IF~~1:10~50
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別名 |
Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
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圖像 |
Anti-HNF4A Antibody (N-term) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-HNF4A Antibody (N-term) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Confocal immunofluorescent analysis of HNF4A Antibody (N-term)( APR07788G) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說明書 |
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數(shù)量 |
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