品牌 |
Leading Biology | 貨號 |
APR15739G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DIAPH2 antibody (Center).
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分子量 |
125569 Da
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細胞定位 |
Antigen Cellular Localization:
Isoform 3: Cytoplasm, cytosol. Early endosome. Note=Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
870-899 aa
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靶點 |
This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 amino acids from the Central region of human DIAPH2.
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亞型 |
Rabbit Ig
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通用名 |
DIA
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基因ID |
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UniProt ID |
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功能 |
Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
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總結(jié) |
DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIAPH2, DIA
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圖像 |
Western blot analysis of DIAPH2 Antibody (Center) (Cat. APR15739G) in 293, MDA-MB435 cell line lysates (35ug/lane). DIAPH2 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. DIAPH2 Antibody (Center) (Cat. APR15739G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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