品牌 |
Leading Biology | 貨號 |
APR15587G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CREM antibody (C-term).
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分子量 |
38940 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
287-314 aa
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靶點(diǎn) |
This CREM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human CREM.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation (PubMed:10373550).
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總結(jié) |
CREM is a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
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別名 |
cAMP-responsive element modulator, Inducible cAMP early repressor, ICER, CREM
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圖像 |
Anti-CREM Antibody (C-term) at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of CREM Antibody (C-term) (Cat. APR15587G) in Hela cell line lysates (35ug/lane). CREM (arrow) was detected using the purified Pab. CREM Antibody (C-term) (Cat. APR15587G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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