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當(dāng)前位置:首頁 > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > GCLM Antibody (C-term)

GCLM Antibody (C-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
AMM04739G 100 μl / ¥4950

GCLM Antibody (C-term)

品牌

Leading Biology

貨號(hào)

AMM04739G

產(chǎn)品分類

Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GCLM antibody (C-term).

分子量

30727 Da

宿主

Rabbit

種屬反應(yīng)性

Human, Mouse

免疫原

246-274 aa

靶點(diǎn)

This GCLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the C-terminal region of human GCLM.

亞型

Rabbit Ig

通用名

GLCLR

基因ID

UniProt ID

總結(jié)

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

儲(chǔ)存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用

WB, IHC-P, FC, IF, E

稀釋方法

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50

別名

Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR

圖像

Anti-GCLM Antibody (C-term) at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GCLM Antibody (C-term) (Cat. AMM04739G) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.

GCLM Antibody (C-term)(Cat. AMM04739G) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

說明書

數(shù)量

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