品牌 |
Leading Biology |
貨號 |
AMM08111G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TBB1 antibody.
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分子量 |
50327 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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靶點 |
This TBB1 antibody is generated from rabbits immunized with human TBB1 recombinant protein.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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總結(jié) |
The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ?tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells.Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ? forms.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Tubulin beta-1 chain, TUBB1
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圖像 |
All lanes : Anti-TBB1 Antibody at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TBB1 Antibody (Cat. AMM08111G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說明書 |
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數(shù)量 |
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