品牌 |
Leading Biology | 貨號 |
APR11601G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CLTA antibody (Center).
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分子量 |
27077 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
99-127 aa
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靶點(diǎn) |
This CLTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human CLTA.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
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總結(jié) |
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Clathrin light chain A, Lca, CLTA
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圖像 |
Western blot analysis of CLTA Antibody (Center) (Cat. APR11601G) in MDA-MB231 cell line lysates (35ug/lane). CLTA (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Western blot analysis of CLTA (arrow) using rabbit polyclonal CLTA Antibody (Center) (Cat. APR11601G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene. |
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說明書 |
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數(shù)量 |
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