品牌 |
Leading Biology | 貨號 |
APR15569G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality COTL1 antibody (C-term).
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分子量 |
15945 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton. Nucleus membrane; Peripheral membrane protein
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
115-142 aa
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靶點 |
This COTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-142 amino acids from the C-terminal region of human COTL1.
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亞型 |
Rabbit Ig
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通用名 |
CLP
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基因ID |
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UniProt ID |
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功能 |
Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.
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總結 |
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Coactosin-like protein, COTL1, CLP
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圖像 |
Western blot analysis of COTL1 Antibody (C-term) (Cat. APR15569G) in Hela cell line lysates (35ug/lane). COTL1 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. COTL1 Antibody (C-term) (Cat. APR15569G) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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