品牌 |
Leading Biology | 貨號 |
APR17569G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NIP7 antibody (C-term).
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分子量 |
20463 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus, nucleolus
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
150-177 aa
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靶點(diǎn) |
This NIP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-177 amino acids from the C-terminal region of human NIP7.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
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總結(jié) |
Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
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別名 |
60S ribosome subunit biogenesis protein NIP7 homolog, KD93, NIP7
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圖像 |
Anti-NIP7 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST. NIP7 Antibody (C-term) (Cat. APR17569G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Western blot analysis of NIP7 (arrow) using rabbit polyclonal NIP7 Antibody (C-term) (Cat. APR17569G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene. |
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說明書 |
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數(shù)量 |
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