HK1 Antibody (C-term)

APR07758G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07758G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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HK1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR07758G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HK1 antibody (C-term).
分子量	102486 Da
細(xì)胞定位	Antigen Cellular Localization: Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	705-734 aa
靶點(diǎn)	This HK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-734 amino acids from the C-terminal region of human HK1.
亞型	Rabbit Ig
基因ID	3098
UniProt ID	P19367
總結(jié)	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1
圖像	Western blot analysis of anti-HK1 Antibody (C-term) (Cat. APR07758G) in A375 cell line lysate. HK1(arrow) was detected using the purified Pab. Western blot analysis of HK1 (arrow) using HK1 Antibody (C-term) (Cat. APR07758G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說(shuō)明書(shū)	Product Datasheet Protocol
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