DGKA Antibody (C-term)

APR15728G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR15728G		100 μl / ￥4950

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品牌	Leading Biology	貨號	APR15728G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DGKA antibody (C-term).
分子量	82630 Da
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	598-628 aa
靶點	This DGKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-628 amino acids from the C-terminal region of human DGKA.
亞型	Rabbit Ig
通用名	DAGK, DAGK1
基因ID	1606
UniProt ID	P23743
功能	Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
總結(jié)	Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha, DGKA, DAGK, DAGK1
圖像	All lanes : Anti-DGKA Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-DGKA Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
數(shù)量

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