MLK4 Antibody (N-term)

APR17393G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR17393G		100 μl / ￥4950

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品牌	Leading Biology	貨號	APR17393G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MLK4 antibody (N-term).
分子量	113957 Da
宿主	Rabbit
種屬反應性	Human
免疫原	33-62 aa
靶點	This MLK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human MLK4.
亞型	Rabbit Ig
基因ID	84451
UniProt ID	Q5TCX8
功能	Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
總結	Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the p38 and JNK signaling cascades. Systematic mutational analyses of tyrosine kinases reveal that a minimum of 30% of colorectal cancer contain at least one mutation in the tyrosine kinases; sampling of colorect cancer cells suggest an important role of MLK4 in this process.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
別名	Mitogen-activated protein kinase kinase kinase MLK4, Mixed lineage kinase 4, MLK4 {ECO:0000303\|Ref1}
圖像	Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody (N-term)(Cat. APR17393G). APR17393G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody (N-term) (Cat. APR17393G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
數(shù)量

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