品牌 |
Leading Biology |
貨號 |
APR17354G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Methionyl tRNA Synthetase antibody (C-term).
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分子量 |
66591 Da
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細胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
564-593 aa
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靶點 |
This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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總結(jié) |
Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet AMP + diphosphate + L-methionyl-tRNAMetThe 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met).This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
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圖像 |
Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-MARS2 Antibody (C-term) (APR17354G) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat. APR17354G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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