CYP3A4 Antibody (Center)

APG02893G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG02893G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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CYP3A4 Antibody (Center)

品牌	Leading Biology	貨號	APG02893G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP3A4 antibody (Center).
分子量	57343 Da
細胞定位	Antigen Cellular Localization: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	228-255 aa
靶點	This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4.
亞型	Rabbit Ig
通用名	CYP3A3
基因ID	1576
UniProt ID	P08684
功能	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide.
總結(jié)	CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
別名	Cytochrome P450 3A4, 11413-, 8-cineole 2-exo-monooxygenase, Albendazole monooxygenase, Albendazole sulfoxidase, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Nifedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase, CYP3A4, CYP3A3
圖像	Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody (Center)( APG02893G). APG02893G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. Western blot analysis of lysates from HepG2, A549, HT-29 cell line, human kidney and liver tissue lysate (from left to right), using CYP3A4 Antibody (Center)( APG02893G). APG02893G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of CYP3A4 Antibody (Center) (Cat. APG02893G) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.
說明書	Product Datasheet Protocol
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