Aromatase (CYP19A1) Antibody (C-term)

APG01994G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APG01994G		100 μl / ￥4950

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Aromatase (CYP19A1) Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APG01994G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Aromatase (CYP19A1) antibody (C-term).
分子量	57883 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Peripheral membrane protein.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	453-485 aa
靶點(diǎn)	This Aromatase (CYP19A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-485 amino acids from the C-terminal region of human Aromatase (CYP19A1).
亞型	Rabbit Ig
通用名	ARO1, CYAR, CYP19
基因ID	1588
UniProt ID	P11511
功能	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
總結(jié)	CYP19A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in the gene encoding CYP19A1 can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
別名	Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19
圖像	Anti-CYP19A1 Antibody (C-term) at 1:1000 dilution + human uterus lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-CYP19A1 Pab (Cat. APG01994G) in Jurkat cell line lysates (35ug/lane).CYP19A1 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human placenta tissue reacted with CYP19A1 antibody (C-term) (Cat. APG01994G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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