品牌 |
Leading Biology |
貨號 |
APR08881G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality OPLAH antibody (N-term).
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分子量 |
137457 Da
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse
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免疫原 |
1-30 aa
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靶點 |
This OPLAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human OPLAH.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
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總結 |
OPLAH catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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別名 |
5-oxoprolinase, 5-oxo-L-prolinase, 5-OPase, Pyroglutamase, OPLAH
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圖像 |
Anti-OPLAH Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 137 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of OPLAH Antibody (N-term) (Cat. APR08881G) in K562, HepG2 cell line lysates and mouse bladder, liver tissue lysates (35ug/lane). OPLAH (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with OPLAH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數量 |
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