品牌 |
Leading Biology | 貨號 |
AMM07020G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality EHD3 antibody (Center).
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分子量 |
60887 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Recycling endosome membrane; Peripheral membrane protein Cytoplasm
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
189-216 aa
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靶點 |
This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the Central region of human EHD3.
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亞型 |
Rabbit Ig
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通用名 |
EHD2, PAST3
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基因ID |
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UniProt ID |
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功能 |
Plays a role in endocytic transport.
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總結(jié) |
EHD3 plays a role in endocytic transport.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
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圖像 |
Western blot analysis of EHD3 Antibody (Center) (Cat. AMM07020G) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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