品牌 |
Leading Biology | 貨號 |
APR11821G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality EN2 antibody (C-term).
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分子量 |
34211 Da
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細胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
243-271 aa
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靶點 |
This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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總結(jié) |
EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2
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圖像 |
Western blot analysis of EN2 Antibody (C-term) (Cat. APR11821G) in mouse cerebellum, brain tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human brain tissue reacted with EN2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. EN2 Antibody (C-term) (Cat. APR11821G) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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