品牌 |
Leading Biology | 貨號 |
APR14961G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ANXA2 antibody (C-term).
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分子量 |
38604 Da
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細胞定位 |
Antigen Cellular Localization:
Secreted, extracellular space, extracellular matrix, basement membrane Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
287-313 aa
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靶點 |
This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-313 amino acids from the C-terminal region of human ANXA2.
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亞型 |
Rabbit Ig
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通用名 |
ANX2, ANX2L4, CAL1H, LPC2D
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基因ID |
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UniProt ID |
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功能 |
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
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總結 |
ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
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別名 |
Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
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圖像 |
Western blot analysis of lysates from A431, SK-BR-3 cell line (from left to right), using ANXA2 Antibody (C-term)(Cat. APR14961G). APR14961G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (C-term) (Cat. APR14961G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Confocal immunofluorescent analysis of ANXA2 Antibody (C-term)( APR14961G) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說明書 |
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數(shù)量 |
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