品牌 |
Leading Biology | 貨號 |
APG03036G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CAMK1G (CaMKI gamma) antibody (C-term).
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分子量 |
53087 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein Cell membrane; Peripheral membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
420-450 aa
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靶點 |
This CAMK1G (CaMKI gamma) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-450 amino acids from the C-terminal region of human CAMK1G (CaMKI gamma).
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亞型 |
Rabbit Ig
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通用名 |
CLICK3, VWS1
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基因ID |
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UniProt ID |
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功能 |
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1 (By similarity).
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總結(jié) |
Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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別名 |
Calcium/calmodulin-dependent protein kinase type 1G, CaM kinase I gamma, CaM kinase IG, CaM-KI gamma, CaMKI gamma, CaMKIG, CaMK-like CREB kinase III, CLICK III, CAMK1G, CLICK3, VWS1
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圖像 |
Western blot analysis of anti-CAMK 1G Pab (Cat. APG03036G) in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G Antibody (C-term) (RB01249). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1G gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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說明書 |
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數(shù)量 |
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