ZPK Antibody (C-term)

APR14304G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR14304G		100 μl / ￥4950

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ZPK Antibody (C-term)

品牌	Leading Biology	貨號	APR14304G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ZPK antibody (C-term).
分子量	93219 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Membrane; Peripheral membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	828-859 aa
靶點(diǎn)	This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-859 amino acids from the C-terminal region of human ZPK.
亞型	Rabbit Ig
通用名	ZPK
基因ID	7786
UniProt ID	Q12852
功能	May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
總結(jié)	ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, DLK, Leucine-zipper protein kinase, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, MAP3K12, ZPK
圖像	Western blot analysis of lysates from HL-60, SH-SY5Y cell line (from left to right), using DLK Antibody(D842)( APR14304G). APR14304G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of anti-ZPK C-term Pab (Cat. APR14304G) in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B). ZPK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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