品牌 |
Leading Biology |
貨號(hào) |
APR17322G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MARK4 antibody (C-term).
|
分子量 |
82520 Da
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human
|
免疫原 |
611-641 aa
|
靶點(diǎn) |
This MARK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 611-641 amino acids from the C-terminal region of human MARK4.
|
亞型 |
Rabbit Ig
|
通用名 |
KIAA1860, MARKL1
|
基因ID |
|
UniProt ID |
|
總結(jié) |
MARK4 contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1-associated kinase domain. RT-PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6-kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony-forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.
|
形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, E
|
稀釋方法 |
WB~~1:1000
|
別名 |
MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1, MARK4, KIAA1860, MARKL1
|
圖像 |
Western blot analysis of anti-MARK4 Pab (Cat. APR17322G) in mouse liver tissue lysate (35ug/lane). MARK4(arrow) was detected using the purified Pab.
Western blot analysis of MARK4 (arrow) using rabbit polyclonal hMARK4-R626 Antibody (Cat. APR17322G).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2) (Origene Technologies).
|
說(shuō)明書(shū) |
|
數(shù)量 |
|