品牌 |
Leading Biology |
貨號 |
APR14330G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HIPK4 antibody (C-term).
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分子量 |
69425 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
537-566 aa
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靶點 |
This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
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總結(jié) |
HIPK4 is a serine/threonine protein kinase that may act as a corepressor of transcription factors.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
Homeodomain-interacting protein kinase 4, HIPK4
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圖像 |
Anti-HIPK4 C-term at 1:1000 dilution + H. testis whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 69 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of HIPK4 C-term (Cat. APR14330G) in HL-60 cell line lysates (35ug/lane). HIPK4 (arrow) was detected using the purified Pab.
Western blot analysis of HIPK4 (arrow) using rabbit polyclonal HIPK4 Antibody (C-term) (Cat. APR14330G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIPK4 gene.
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說明書 |
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數(shù)量 |
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