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當(dāng)前位置:首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > GRK1 Antibody (C-term)

GRK1 Antibody (C-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢(xún)價(jià)
APR12283G 100 μl / ¥4950

GRK1 Antibody (C-term)

品牌

Leading Biology

貨號(hào)

APR12283G

產(chǎn)品分類(lèi)

Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GRK1 antibody (C-term).

分子量

63526 Da

細(xì)胞定位

Antigen Cellular Localization: Membrane {ECO:0000250|UniProtKB:P28327}; Lipid-anchor {ECO:0000250|UniProtKB:P28327}

宿主

Rabbit

種屬反應(yīng)性

Human, Mouse

免疫原

520-550 aa

靶點(diǎn)

This GRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human GRK1.

亞型

Rabbit Ig

通用名

RHOK

基因ID

UniProt ID

功能

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

總結(jié)

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

儲(chǔ)存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用

WB, IHC-P, E

稀釋方法

WB~~1:1000 IHC-P~~1:50~100

別名

Rhodopsin kinase, RK, G protein-coupled receptor kinase 1, GRK1, RHOK

圖像

The anti-GRK1 Pab (Cat. APR12283G) is used in Western blot to detect GRK1 in HeLa cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK1 Antibody (C-term) (Cat. APR12283G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

說(shuō)明書(shū)

數(shù)量

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