FTO Antibody (N-term)

AMM04628G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM04628G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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FTO Antibody (N-term)

品牌	Leading Biology	貨號	AMM04628G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FTO antibody (N-term).
分子量	58282 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Nucleus speckle
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1-30 aa
靶點	This FTO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FTO.
亞型	Rabbit Ig
通用名	KIAA1752
基因ID	79068
UniProt ID	Q9C0B1
功能	Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
總結(jié)	The precise function of FTO remains to be determined.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
別名	Alpha-ketoglutarate-dependent dioxygenase FTO, 11411-, Fat mass and obesity-associated protein, FTO, KIAA1752
圖像	Western blot analysis of FTO Antibody (N-term) (Cat. AMM04628G) in NCI-H460 cell line lysates (35ug/lane). FTO (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human brain tissue reacted with FTO Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of NCI-H292 cells using FTO Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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