品牌 |
Leading Biology |
貨號 |
APR08632G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ND5 antibody (C-term).
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分子量 |
67027 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion inner membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
544-570 aa
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靶點 |
This ND5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-570 amino acids from the C-terminal region of human ND5.
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亞型 |
Rabbit Ig
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通用名 |
MTND5, NADH5, ND5
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基因ID |
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UniProt ID |
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功能 |
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
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總結(jié) |
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5, MT-ND5, MTND5, NADH5, ND5
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圖像 |
All lanes : Anti-ND5 Antibody (C-term) at 1:1000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of CEM cells using ND5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說明書 |
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數(shù)量 |
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