品牌 |
Leading Biology |
貨號 |
APR10518G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TPH1 antibody (N-term).
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分子量 |
50985 Da
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
35-62 aa
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靶點 |
This TPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human TPH1.
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亞型 |
Rabbit Ig
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通用名 |
TPH, TPRH, TRPH
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基因ID |
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UniProt ID |
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總結 |
TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, IF, E
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稀釋方法 |
WB~~1:500
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
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別名 |
Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH
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圖像 |
Anti-TPH1 Antibody (N-term) at 1:500 dilution + 293 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue with TPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of widr cells using TPH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說明書 |
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數(shù)量 |
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