品牌 |
Leading Biology | 貨號(hào) |
APR12743G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PAX8 antibody (Center).
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分子量 |
48218 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
285-313 aa
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靶點(diǎn) |
This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
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總結(jié) |
PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this family contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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別名 |
Paired box protein Pax-8, PAX8
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圖像 |
All lanes : Anti-PAX8 Antibody (Center) at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of PAX8 Antibody (Center) (Cat. APR12743G) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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說(shuō)明書(shū) |
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數(shù)量 |
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