品牌 |
Leading Biology | 貨號(hào) |
APR15842G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ENTH antibody (Center).
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分子量 |
68259 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
222-249 aa
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靶點(diǎn) |
This ENTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-249 amino acids from the Central region of human ENTH.
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亞型 |
Rabbit Ig
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通用名 |
ENTH, EPN4, EPNR, KIAA0171
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基因ID |
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UniProt ID |
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功能 |
Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
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總結(jié) |
ENTH binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). It May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. It stimulates clathrin assembly.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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別名 |
Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Clint, Enthoprotin, Epsin-4, Epsin-related protein, EpsinR, CLINT1, ENTH, EPN4, EPNR, KIAA0171
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圖像 |
Western blot analysis of ENTH Antibody (Center) (Cat. APR15842G) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab. (LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說(shuō)明書(shū) |
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數(shù)量 |
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