品牌 |
Leading Biology | 貨號 |
APR17108G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality KRT4 antibody (C-term).
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分子量 |
57285 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
386-415 aa
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靶點 |
This KRT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of human KRT4.
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亞型 |
Rabbit Ig
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通用名 |
CYK4
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UniProt ID |
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總結(jié) |
The protein is a member of the keratin family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4
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圖像 |
Western blot analysis of KRT4 antibody (C-term) (Cat. APR17108G) in mouse spleen tissue lysates (35ug/lane). KRT4 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with KRT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of MDA-231 cells using KRT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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