品牌 |
Leading Biology | 貨號 |
AMM07077G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PDIA6 antibody (Center D251).
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分子量 |
48121 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138}. Cell membrane. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
236-264 aa
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靶點(diǎn) |
This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human PDIA6.
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亞型 |
Rabbit Ig
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通用名 |
ERP5, P5, TXNDC7
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基因ID |
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UniProt ID |
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功能 |
May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
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總結(jié) |
Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7
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圖像 |
Western blot analysis of PDIA6 Antibody (Center D251) (Cat. AMM07077G) in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center D251), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of hela cells using PDIA6 Antibody (Center D251)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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