品牌 |
Leading Biology | 貨號 |
AMM07016G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality EEF1D antibody (N-term).
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分子量 |
31122 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Isoform 2: Nucleus
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
1-30 aa
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靶點 |
This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.
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亞型 |
Rabbit Ig
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通用名 |
EF1D
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基因ID |
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UniProt ID |
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功能 |
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
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總結(jié) |
EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D
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圖像 |
Western blot analysis of EEF1D antibody (N-term) (Cat. AMM07016G) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. EEF1D Antibody (N-term) (Cat. AMM07016G) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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