Tuberin (TSC2) Antibody (S1798)

APR10606G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR10606G		100 μl / ￥4950

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Tuberin (TSC2) Antibody (S1798)

品牌	Leading Biology	貨號(hào)	APR10606G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Tuberin (TSC2) antibody (S1798).
分子量	200608 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1776-1805 aa
靶點(diǎn)	This Tuberin (TSC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1776-1805 amino acids from human Tuberin (TSC2).
亞型	Rabbit Ig
通用名	TSC4
基因ID	7249
UniProt ID	P49815
功能	In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
總結(jié)	Mutations in TSC2 lead to tuberous sclerosis complex. This protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. It may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
別名	Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4
圖像	Western blot analysis of lysates from PC-3, LNCaP, Hela cell line (from left to right), using Tuberin (TSC2) Antibody( APR10606G). APR10606G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, Hela, cell line (from left to right), using Tuberin (TSC2) Antibody( APR10606G). APR10606G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Fluorescent confocal image of Hela cell stained with Tuberin (TSC2) Antibody (S1798)( APR10606G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.
說(shuō)明書(shū)	Product Datasheet Protocol
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