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當(dāng)前位置:首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > Leptin Receptor (LEPR) Antibody (N-term)

Leptin Receptor (LEPR) Antibody (N-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢(xún)價(jià)
APR08200G 100 μl / ¥4950

Leptin Receptor (LEPR) Antibody (N-term)

品牌

Leading Biology

貨號(hào)

APR08200G

產(chǎn)品分類(lèi)

Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Leptin Receptor (LEPR) antibody (N-term).

分子量

132494 Da

細(xì)胞定位

Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein

宿主

Rabbit

種屬反應(yīng)性

Human

免疫原

23-52 aa

靶點(diǎn)

This Leptin Receptor (LEPR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human Leptin Receptor (LEPR).

亞型

Rabbit Ig

通用名

DB, OBR

基因ID

UniProt ID

功能

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).

總結(jié)

Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR). LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

儲(chǔ)存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用

WB, FC, IHC-P, E

稀釋方法

WB~~1:1000 FC~~1:10~50 IHC-P~~1:50~100

別名

Leptin receptor, LEP-R, HuB219, OB receptor, OB-R, CD295, LEPR, DB, OBR

圖像

Leptin Receptor (LEPR) Antibody (N-term)(Cat. APR08200G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).

Leptin Receptor (LEPR) Antibody (N-term) (Cat. APR08200G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

說(shuō)明書(shū)

數(shù)量

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