品牌 |
Leading Biology | 貨號 |
APG01404G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ABCB10 antibody (N-term).
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分子量 |
79148 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion inner membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
1-27 aa
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靶點(diǎn) |
This ABCB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human ABCB10.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
May mediate critical mitochondrial transport functions related to heme biosynthesis.
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總結(jié) |
The membrane-associated protein ABCB10 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
IHC-P~~1:10~50
WB~~1:1000
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別名 |
ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10, ABC transporter 10 protein, Mitochondrial ATP-binding cassette 2, M-ABC2, ABCB10
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圖像 |
APG01404G staining hABCB10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-ABCB10 Antibody (S16) at 1:1000-1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 79 kDa Blocking/Dilution buffer: 5% NFDM/TBST. ABCB10 Antibody (S16) (Cat. APG01404G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ABCB10 antibody detected the ABCB10 protein (arrow). |
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說明書 |
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數(shù)量 |
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