品牌 |
Leading Biology | 貨號 |
APR15524G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CLTC antibody (Center).
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分子量 |
191615 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
1019-1048 aa
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靶點(diǎn) |
This CLTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1048 amino acids from the Central region of human CLTC.
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亞型 |
Rabbit Ig
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通用名 |
CLH17, CLTCL2, KIAA0034
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基因ID |
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UniProt ID |
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功能 |
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLTC, CLH17, CLTCL2, KIAA0034
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圖像 |
Anti-CLTC Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 192 kDa Blocking/Dilution buffer: 5% NFDM/TBST. CLTC antibody (Center) (Cat. APR15524G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. CLTC Antibody (Center) (Cat. APR15524G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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