品牌 |
Leading Biology |
貨號 |
APR13326G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SLC12A5 antibody (N-term).
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分子量 |
126184 Da
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細胞定位 |
Antigen Cellular Localization:
Membrane; Multi-pass membrane protein.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
5-33 aa
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靶點 |
This SLC12A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human SLC12A5.
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亞型 |
Rabbit Ig
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通用名 |
KCC2, KIAA1176
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基因ID |
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UniProt ID |
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功能 |
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
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別名 |
Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, hKCC2, Neuronal K-Cl cotransporter, SLC12A5, KCC2, KIAA1176
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圖像 |
Anti-SLC12A5 Antibody (N-term) at 1:4000 dilution + human brain lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 126 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
SLC12A5 Antibody (N-term) (Cat. APR13326G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A5 antibody detected the SLC12A5 protein (arrow).
SLC12A5 Antibody (N-term) (Cat. APR13326G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC12A5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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