品牌 |
Leading Biology | 貨號 |
AMM04644G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality G6PD antibody (Center).
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分子量 |
59257 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
297-326 aa
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靶點(diǎn) |
This G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human G6PD.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
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總結(jié) |
G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Glucose-6-phosphate 1-dehydrogenase, G6PD, G6PD
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圖像 |
Western blot analysis of G6PD Antibody (Center) (Cat. AMM04644G) in K562,MCF-7 cell line lysates (35ug/lane).G6PD (arrow) was detected using the purified Pab. G6PD Antibody (Center) (Cat. AMM04644G) IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. G6PD Antibody (Center) (Cat. AMM04644G) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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