品牌 |
Leading Biology | 貨號 |
APG01959G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AQP1 antibody.
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分子量 |
28; [uniprot]28,20,23,17; KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat, Dog, Sheep
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免疫原 |
181-269/269 aa
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通用名 |
CHIP28
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基因ID |
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UniProt ID |
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功能 |
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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總結(jié) |
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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形式 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB
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稀釋方法 |
WB~~1:100~1:500
IHC-P~~1:100~1:500
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別名 |
Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28
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圖像 |
Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37?C. Predicted band 28kD. Observed band size: 28kD. Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (APG01959G) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-AF488) at [1:500] for 90 minutes and DAPI staining of the nuclei. |
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說明書 |
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數(shù)量 |
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