PACAP-27/38 Antibody

APR12700G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR12700G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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PACAP-27/38 Antibody

品牌	Leading Biology	貨號	APR12700G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PACAP-27/38 antibody.
分子量	19; [uniprot]19; [RD]19; KDa
細(xì)胞定位	Antigen Cellular Localization: Secreted.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse, Rat
免疫原	132-169/176 aa
基因ID	116
UniProt ID	P18509
功能	Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.
總結(jié)	Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.
形式	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC
稀釋方法	WB~~ 1:500 IHC~~1:100-1:500
別名	Pituitary adenylate cyclase-activating polypeptide, PACAP, PACAP-related peptide, PRP-48, Pituitary adenylate cyclase-activating polypeptide 27, PACAP-27, PACAP27, Pituitary adenylate cyclase-activating polypeptide 38, PACAP-38, PACAP38, ADCYAP1
圖像	Western blot analysis of lysate from human brain tissue lysate,using PACAP-27/38 Antibody(APR12700G). APR12700G was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PACAP-27/38 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
說明書	Product Datasheet Protocol
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