品牌 |
Leading Biology | 貨號(hào) |
APR08838G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NSE antibody.
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分子量 |
47 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat
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免疫原 |
392-425 aa
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基因ID |
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UniProt ID |
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功能 |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
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總結(jié) |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
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形式 |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, IHC
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稀釋方法 |
WB~~ 1:1000
IF~~1:100
IHC~~1:50-1:100
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別名 |
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
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圖像 |
Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Western blot analysis of extracts from Hela cells (Lane 1), colo cells(Lane 2),HepG2 cells(Lane 3) and 293 cells (Lane 4), using NSE Antibody. The lane on the left is treated with synthesized peptide. Immunofluorescence analysis of A549 cells, using NSE antibody . |
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說(shuō)明書(shū) |
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數(shù)量 |
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